日本不卡中文字幕免费-日本不无在线一区二区三区-日本成a人片在线播放-日本成a人片在线播放神马在线播放-日本成a人片在线观看影院-日本成本人片视频免费

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動物上皮細(xì)胞>CCL-64Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞

Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞

簡要描述:CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號:CCL-64
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2968

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細(xì)胞 的詳細(xì)介紹

CCL-64

ATCC® Number:CCL-64™  Price:$275.00
Designations:Mv 1 Lu (NBL-7)

Depositors:AJ Kniazeff

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Musa vison (mink)

Morphology:epithelial


Source:Organ: lung

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Isolation:Isolation date: May, 1964

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Resistance:adenovirus 5; coxsackievirus A9, B5; poliovirus 2

Cytogenetic Analysis:Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.

Age:near term fetus

Gender:male and female mixed

Comments:The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink.The cells are useful for focus forming assays for murine and feline sarcoma viruses [PubMed: 4366800].

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

purified DNA:ATCC CCL-64D

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 羞羞影院午夜男女爽爽免费 | 伊人网视频在线观看 | 精品午夜国产福利在线观看 | 成人性生交大片免费看r | 日本一区二区三区免费在线观看 | 91久久国产口精品久久久久 | 中文字幕有码中文无码 | 99精品久久99久久久久 | 日韩美女拍拍免费视频网站 | 中文字幕精品无码热在线视频 | 亚洲全黄无码在线观看 | 国产精品成人嫩草影院 | 91精品福利在线观看 | 午夜精品久久久久久久妓女 | 午夜十二点免费在线播放 | 午夜视频在线观看一区二区 | 亚洲国产亚洲片在线观看播放 | 日本九九热在线观看官网 | 国产精品亚洲αv天堂无码 国产精品亚洲а∨天堂网不卡 | 亚洲久悠悠色悠在线播放 | 久久精品国产亚洲av香蕉高清 | 欧美日韩国产成人综合在线影院 | 99精品热在线在线观看视频 | 91亚洲国产成人久久精品蜜臀 | 亚洲国产亚洲综合在线尤物 | 最新剧情片大全免费在线观看 | 亚洲中文字幕欧美自拍 | 福利姬国产精品一区在线 | 最新男人必看的私人影院高清在线手机观看 | 精品成人毛片在线观看 | 国产不卡av在线免费观看 | 综合网永久入口 | 91久久久久| 国产一区二区三区在线2025 | 日韩欧美一区二区三区在线观 | 人妻系列一区二区三区 | 无码av中文一区 | 国产av毛片影院精品资源 | 尤物视频一区二区在线观看 | 91麻豆精品久久久久蜜臀 | 青青国产成人 |