日本不卡中文字幕免费-日本不无在线一区二区三区-日本成a人片在线播放-日本成a人片在线播放神马在线播放-日本成a人片在线观看影院-日本成本人片视频免费

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-3006JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

簡要描述:CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-3006
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-15
  • 訪  問  量:2073

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

ATCC® Number:CRL-3006™    Price:$417.00
Designations:JeKo-1

Depositors:Elias Campo

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Homo sapiens (human)

Morphology:lymphoblast

Characterization Data



Source:Organ: peripheral blood
Tissue: Mantle Cell Lymphoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

Antigen Expression:CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

DNA Profile (STR):Amelogenin:X

CSF1PO:9,12

D13S317:8,9

D16S539:12

D5S818:10,13

D7S820:10,11

THO1:7

TPOX:8

vWA: 14



Age:78

Gender:female

Comments:The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C


Subculturing:Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

Preservation:Freeze medium: 30% RPMI; 60% FBS; 10% DMSO
Storage temperature: liquid nitrogen vapor phase


References:92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

CRL-3006

ATCC® Number:CRL-3006™    Price:$417.00
Designations:JeKo-1

Depositors: Elias Campo

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Homo sapiens (human)

Morphology:lymphoblast

Characterization Data



Source:Organ: peripheral blood

Tissue: Mantle Cell Lymphoma



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Antigen Expression:CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

DNA Profile (STR):Amelogenin:X

CSF1PO:9,12

D13S317:8,9

D16S539:12

D5S818:10,13

D7S820:10,11

THO1:7

TPOX:8

vWA: 14



Age:78

Gender:female

Comments:The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: 5% CO2 in air recommended

Temperature: 37.0°C



Subculturing:Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

Preservation:Freeze medium: 30% RPMI; 60% FBS; 10% DMSO

Storage temperature: liquid nitrogen vapor phase



References:92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 国产91久久九九免费精品无码 | 99国产欧美久久久精品蜜桃 | 亚洲精品白浆高清久久久久久 | 亚洲免费观看视频 | 2025精品国产自产拍在线观看 | 日本高清中文字幕在线 | 午夜成人精品福利网站在线观看 | 精品久久国产精品 | 午夜十二点免费在线播放 | 欧美一级片播放 | 麻豆精品一区二区综合av | 成人精品 | 日韩中文人妻无码不卡 | 水蜜桃成视频人在线播放 | 国产在线视频国产永久 | 熟妇精品区| 91精品夜夜夜一区二区 | 国产日韩欧美高清一区二区三区 | 色综合久久婷婷五月91 | 日韩精品无码一区二区三区视频 | 天堂网无码av手机版 | 国产在线观看三级高清 | 91大神在线资源观看无广告 | 精品三级av无码一区 | 人人揉人人爽五月天视频 | 97久久精品人人做人人爽 | 国产精品午夜小视频观看 | 国产精品一区二区精品视频 | 亚洲最大中文字幕无码网站 | 久久精品麻豆日日躁夜夜躁妓女 | 欧美在线一 | 91久久精一区 | 欧美日韩亚洲综合一区二区三区激情在线 | 少妇午夜福利一区二区 | 午夜免费视频 | 国产精品精品 | 国产无遮挡又黄又大又爽 | 亚洲综合网站精品一区二区 | 国产精品一区二区三区四区五区上线网友表示 | 91精品啪在线观看国产线免费 | 国产剧美女开车视频网站 |